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      promega,M-MLV Reverse Transcriptase,M1701

      描述:上海起發(fā)簽約promega

      更新時間:2015-06-18
      訪問次數(shù):3189
      廠商性質(zhì):代理商
      詳情介紹

      M-MLV Reverse Transcriptase
      ·                                 Protocol
      ·                                 MSDS
      ·                                 Components
      M-MLV Reverse Transcriptase
      ·                                 M-MLV Reverse Transcriptase
      ·                                 M-MLV RT 5X Buffer
      10,000u
      200u/μl
      M1701

       
      QC Tests
      Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, first-strand cDNA synthesis.
      Source
      Recombinant E. coli strain.
      Storage Buffer
      20mM Tris-HCl (pH 7.5 at 25°C), 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.
      Storage Conditions
      Store at –20°C.
      Unit Definition
      One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H]dTTP, 0.025mM oligo(dT), 0.25mM poly(A) and 0.01% NP-40.
      For product intended use please see Patents & Disclaimers tab.

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